rabbit anti rat asic2a antibody  (Alomone Labs)

Journal: Neural Regeneration Research

Article Title: Ischemic postconditioning protects against ischemic brain injury by up-regulation of acid-sensing ion channel 2a

doi: 10.4103/1673-5374.180751

Figure Lengend Snippet: ASIC2a protein expression in the rat hippocampal CA1 region. Ischemic postconditioning strongly up-regulated ASIC2a protein expression in the rat hippocampal CA1 region after ischemic brain injury. ASIC2a: Acid-sensing ion channel 2a; GAPDH: glyceraldehye phosphate dehydrogenase.

Article Snippet: Membranes were blocked using 5% skimmed milk, incubated at room temperature for 120 minutes, washed four times (10 minutes each) in PBS-Tween, incubated with primary rabbit anti-rat ASIC2a and GAPDH polyclonal antibodies (1:5,000; Sigma, St Louis, MO, USA) at 4°C overnight, washed four times (10 minutes each) in PBS-Tween, incubated with horseradish peroxidase-labeled goat anti-rabbit IgG (1:2,000; Sigma) at room temperature for 120 minutes, and washed four times (10 minutes each) in PBS-Tween.

Techniques: Expressing

Journal: Neural Regeneration Research

Article Title: Ischemic postconditioning protects against ischemic brain injury by up-regulation of acid-sensing ion channel 2a

doi: 10.4103/1673-5374.180751

Figure Lengend Snippet: ASIC2a mRNA expression in the rat hippocampal CA1 region in both groups

Article Snippet: Membranes were blocked using 5% skimmed milk, incubated at room temperature for 120 minutes, washed four times (10 minutes each) in PBS-Tween, incubated with primary rabbit anti-rat ASIC2a and GAPDH polyclonal antibodies (1:5,000; Sigma, St Louis, MO, USA) at 4°C overnight, washed four times (10 minutes each) in PBS-Tween, incubated with horseradish peroxidase-labeled goat anti-rabbit IgG (1:2,000; Sigma) at room temperature for 120 minutes, and washed four times (10 minutes each) in PBS-Tween.

Techniques: Expressing